The Thermo Scientific Listeria Precis Methods “the most efficient culture-based workflows for testing foods and environmental samples for Listeria”
BASINGSTOKE, UK (November 30, 2022) – Faster, simpler, more robust, and more reliable: the next generation of culture media-based testing for detection and enumeration of Listeria is here, promising to drive productivity and boost profitability in the competitive world of pathogen testing.
“The Thermo Scientific Listeria Precis Methods provide laboratories with three simple steps to a confirmed Listeria test result lowering test per cost compared to traditional culture media-based and immunoassay methods including providing the fastest time to actionable result (next to PCR) for Listeria species and L. monocytogenes”. According to Jaakko McVey, global senior marketing manager, Thermo Fisher Scientific.
“This is all within a simple, easy-to-use workflow with a strong validation footprint. It adds up to dramatically streamlined laboratory operations, faster product release, and higher confidence in results.”
Listeria Precis Methods are validated for a broad range of samples according to ISO 16140-2 following NF Validation rules when compared to the ISO 11290-1:2017 and ISO 11290-2:2017 reference methods for the detection and enumeration of Listeria species and L. monocytogenes. The Listeria Precis Detection Method requires a single, rapid, enrichment and utilizes our newest and most advanced chromogenic agar plate; the Thermo Scientific Brilliance Listeria Agar (ISO). Brilliance Listeria Agar has been demonstrated to enable detection of Listeria-positive samples at
22 hours with, market leading, high specificity, that minimizes unnecessary colony confirmation. Confirmation of presumed-positive colonies then takes just 15 minutes using a new rapid method for confirming Listeria species or Listeria monocytogenes direct from the plate, leading to same-day resolution of positive and negative samples. Faster confirmation allows for a higher operational throughput and release of Listeria-free products, maximizing profit margins.
A further development has enabled a simple 10 μL inoculation of plates from the enrichment broth, rather than 100 μL with a pipette. This significantly reduces the handling time and the risk of
cross-contamination. In total it is estimated a laboratory can gain a 33% reduction in handling time during screening.
How it works:
Simpler handling – 33% reduction in plate inoculation time
Faster detection – negative and confirmed-positive results in just over 42 hours
Improved accuracy – outperforms other rapid culture and immunological methods
Enumeration of Listeria species and L. monocytogenes using the Listeria Precis Enumeration Method takes around 24 hours for most samples, followed by a 15-minute count and confirmation step when suspect colonies are detected. The advanced Brilliance Listeria Agar is also compatible with the ISO 11290-1 and ISO 11290-2 workflows for the detection and enumeration of Listeria.
Daniele Sohier, senior manager, global scientific affairs at Thermo Fisher, said: “The Listeria Precis Methods bring a couple of wins that make a dramatic improvement for Listeria detection. Improved specificity and handling have a significant impact on resource management achieved through time savings. The method is extremely reliable, reducing the number of presumptive-positive colonies to be confirmed and reducing confirmation time from six hours to two days or more, down to just 15 minutes.”
“This a game-changer for food testing laboratories, especially those working in time-limited settings, where certain products can only be released to market after a negative test. Also, for ready-to-eat foods, which can present a higher risk of harboring the resilient Listeria bacteria.”
For more information visit thermofisher.com/listeria-precis