Page 94 - FoodFocusThailand No.199 October 2022

STRONG QC & QA

aerobic and anaerobic bacteria, mycobacteria, yeast, and mold. halal foods, etc. For FT-IR spectroscopy in biological science,
For food microbiological research, MALDI-TOF MS has been the mid-IR spectrum (between 4,000 and 400 cm , or from
−1
used for the identification of foodborne pathogens and food 2.5 to 25 mm.) was the most useful frequency. It has been
spoilage microorganisms, including filamentous fungi, yeast, applied in diverse microbiological contexts and for different
and bacteria in various food products; for example, beverages, purposes. One of the most explored applications is for
honey, dairy products, processed-meat products, and seafood. discrimination, classification, and identification of bacteria at
Furthermore, it can be used to identify and type microorganisms different taxonomic levels, including genera, species,
isolated from the production environment, which is a crucial serotype/serogroup, and strain levels, to investigate
part of preventive control measures entitled environmental microbiological source tracking. Furthermore, FT-IR
monitoring. spectroscopy can identify the changes in the molecular
composition of microbial cells. It can monitor biomarkers of
FT-IR bacteria cells and biomass composition to quantify bacteria.
General information Fourier Transform Infrared (FT-IR) This information can describe the response of bacterial cells
spectroscopy is a biophysical technique to analyze the functional against stress exposure, such as heat, acids, or antimicrobial
groups of molecules, providing information on molecular compounds, and predict microbial growth/destruction. Thus,
structures and performing a unique and specific spectrum. This the application of FTIR spectroscopy in food microbiology is
information can provide in both qualitative and quantitative growing and has the potential to be used further in food
analyses. FT-IR spectroscopy is frequently used for organic microbiological research and the food industry.
samples, either in solid or liquid forms. It is a rapid, simple,
non-destructive, chemical-free, cost-efficient, and high-
performance technique. Therefore, it is widely applied in different
advanced research areas, for example, material sciences, More Information Service Info C011
biochemistry, medical science, and biological science. Lately,
its performance has been further enhanced by connecting mass
spectroscopy to the microscope, called FT-IT imaging, to เอกสารอ้างอิง / References
analyze the small sample and create a chemical mapping. กรมวิิทยาศาสตร์การแพทย์ กระทรวิงสาธารณสุข. 2559. กรมวิิทย์ฯ ใช้้
Furthermore, the use of synchrotron-source FT-IT เทคนิิคใหม่ตรวิจจำาแนิกเช้้�อแบคทีเรีย/เช้้�อรา ร้้ผลเร็วิ ถู้กต้อง แม่นิยำา.
microspectroscopy offers an effective method to characterize ข่าวิกรมวิิทยาศาสตร์การแพทย์, 25 พฤษภาคม 2559.
the chemical composition across surfaces with high resolution, วิิยดา กวิานิเหียนิ. 2556. เคร้�องจำาแนิกแบคทีเรียอัตโนิมัติในิระบบแมสสเปก
โทรเมตรีช้นิิด MALDI-TOF. วิารสารเทคนิิคการแพทย์และกายภาพบำาบัด.
consequently accuracy and reliable information. 25 (2): 126-131.
Principle and function IR spectroscopy relies on the ศิรินิิตย์ ธารธาดา และ ปฐมาพร อำานิาจอนิันิต์. 2561. เทคโนิโลยี Fourier
absorption of specific wavelengths within the IR spectra upon Transform Infrared Spectroscopy: ทฤษฎีีและการประยุกต์ใช้้. วิารสาร
interacting with certain molecular bonding. IR radiation is วิิทยาศาสตร์สุขภาพสัตวิ์และเทคโนิโลยี. 2 (1): 29-33
absorbed by certain molecules and converted into energy of Akimowicz, M. and J. Bucka-Kolendo. 2020. MALDI-TOF MS – ap
molecular vibration. These modes of vibration are different, plication in food microbiology. Acta Biochimica Polonica. 67 (3):
including stretching (symmetry and asymmetry) and bending 327-332.
(scissoring, rocking, wagging, and twisting), and depend on the Höll, L., J. Behr and R.F. Vogel. 2016. Identification and growth dy
chemical bonds and functional groups, for example, double namics of meat spoilage microorganisms in modified atmosphere
bond, triple bond, carbonyl group, hydroxyl group or amino packaged poultry meat by MALDI-TOF MS. Food Microbiol, 60,
group, comprising in the molecules. These informational signals 84-91.
are measured and transferred to the computer to create a Moran-Gilad J. and Y. Yagel. 2021. Application and Integration of
Omics-powered Diagnostics in Clinical and Public Health Micro
Fourier transformation of the signal. Then the Fourier biology.
transformation of the signal is calculated by a mathematical Naksang, P., S. Tongchitpakdee, K. Thumanu, M.J. Oruna-Concha,
procedure and interpreted as the spectrum specific to molecular K. Niranjan and C. Rachtanapun. 2020. Assessment of antimicro
composition. The IR region are between 13,000 and 10 cm −1 bial activity, mode of action and volatile compounds of Etlingera
(wavelengths from 0.78 to 1,000 mm.), dividing into three ranges pavieana essential oil. Molecules. 25 (14): 3245.
such as near-IR (4,000 – 12,800 cm ), mid-IR (200 - 4,000 Sangnark, K. and C. Rachtanapun. 2018. Identification of spoilage
-1
cm ) and far- IR (10 – 200 cm ). microorganisms in ready-to-eat sausage by MALDI-TOF MS in
-1
-1
Application of FT-IR in food industry and food Proceedings of the 20th Food Innovation Asia Conference (FIAC
microbiology Fourier-transform infrared (FTIR) spectroscopy 2018) Creative Food for Future and Sustainability, p 366-371, BITEC,
is widely applied for routine quality control, safety assessment, Bangkok Thailand. 14-16 June 2018.
and authentication in food industries, for example, detecting Tantala, J., K. Thumanu and C. Rachtanapun. 2019. An assessment
moisture content in soybean, chemical composition analysis of of antibacterial mode of action of chitosan on Listeria innocua cells
using real-time HATR-FTIR spectroscopy. International Journal of
raw milk, detection of food adulteration in fish sauce, honey, Biological Macromolecules. 135: 386-393.

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