Page 117 - FoodFocusThailand No.210 SEPTEMBER 2023

STAND OUT TECHNOLOGY

that produce the most stable refolded pipettes for their assays. They immediately noticed the more comfortable pipetting
d
protein with the highest melting experience and the high-quality data provided by modern technology . Confident
c
temperature. By screening out suboptimal that these multichannel pipettes would improve throughput and data quality and
d
refolding conditions, native structure(s) diminish the risk of injuries.
can be identified quickly and, more Pipettes are very important scientific equipment for research laboratories. It
specifically, characterized by further is often used for dosing-measurement of liquid samples in chemical and biological
experimentation. laboratories for use in the food industry, medicine, and medical. A multichannel
For accurate data, DGR assays must pipette is a small-volume dosing device for applications that require high precision
be prepared quickly and carefully. Mark and accurate results. It also reduces sample loss and reduces operator fatigue.
Lee, a technician in the Phillips lab, In addition to choosing a suitable pipette, it is essential for the user to study the
highlights two potential pitfalls: first, to technique of pipette usage to minimize experimental errors that may occur.
screen many refolding conditions,
particularly in 384-well format, reagents
must be dispensed with the highest
precision and accuracy. Secondly, DGR More Information Service Info C017
relies on fluorescence from the light-
sensitive compound orange . Mr. Lee
a
described the lab’s attempts to refine their
experiments. Using single-channel
pipettes consumed 10 minutes per 96-well
plate and produced inconsistent data due
to fading of the orange signal. Multichannel
a
pipettes improved throughput, but the lab’s
existing instruments caused user fatigue
and risked repetitive strain injuries.
Moreover, researchers were concerned
about the repeatability from tip to tip of the
volumes aspirated and sample loss when
tips fell off.
Multichannel pipettes: the Easy
Way to Increase Efficiency of
Testing
Mr. Lee discussed options with the
expert lab’s representative to find out the
b
right solution. Familiar with their single-
channel pipettes and modern technology ,
c
the lab chose to demo these multichannel
ข้้อมููลเพิ่่�มูเติ่มู / Additional information
a SYPRO Orange เป็็นสีีย้้อมเรืืองแสีงป็รืะเภท
เมโรืไซย้านีน เพื่ื�อใช้้ในการืรืะบุุคุุณลัักษณะของ
โป็รืตีีน
SYPRO Orange is a merocyanine -type
fluorescent dye that is used for protein
staining in the course of protein characterization.
b บุรืิษัท เมทเลั่อรื์-โทเลัโด (ป็รืะเทศไทย้) จำำากัด
Mettler-Toledo (Thailand) Co., Ltd.
c LiteTouch™ System (LTS™) เทคุโนโลัย้ีที�ช้่วย้
ลัดแรืงในการืโหลัดแลัะดีดทิป็ จำึงช้่วย้ลัดคุวาม
เมื�อย้ลั้าจำากการืใช้้งานป็ิเป็ตี จำาก บุรืิษัท เมทเลั่อรื์-
โทเลัโด (ป็รืะเทศไทย้) จำำากัด
LiteTouch™ System (LTS™) reduces the
amount of force required to load and eject
tips to less fatigue from pipettes from
Mettler-Toledo (Thailand) Co., Ltd.
TM
d Rainin Multichannel pipettes จำาก
บุรืิษัท เมทเลั่อรื์-โทเลัโด (ป็รืะเทศไทย้) จำำากัด
TM
Rainin Multichannel pipettes from
Mettler-Toledo (Thailand) Co., Ltd.
เอกสารอ้างอ่ง / Reference
1 Biter, A., et al. 2016. DSF Guided Refolding
as a Novel Method of Protein Production.
Scientific Reports 6: 18906
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