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STAND OUT TECHNOLOGY
97
JUN 2017 FOOD FOCUSTHAILAND
The quality assurance plays a significant role in analyzing and
monitoring of such safety for consumers. And since UHT products
canbe consumedwhenever theywant, the inspectionagency need
toworkagainst time toensure thatUHTproductswill bedelivered to
customers in time,butstillmaintain thegoodquality inspectionbefore
theproductsarehanded tocustomersorconsumers.However, rapidly
testing report not only helps manufacturers have greater business
opportunities by releasing products to the market, but it can also
reduce the storage space and amount of inventory. This also helps
reduce cost in overall including labor cost, inventory management
cost, and business opportunity cost, andetc.
TheMicroorganismAnalysisMethod
for UHTProducts
In general, themethod of microorganism analysis inUHT products
is divided into two steps. The first step is the pre-incubation. It is an
important step for UHT-based products because this system can
destroymicroorganisms,pathogensand foodspoilagemicroorganisms
that grow rapidly at room temperature.As a result, even there is no
microorganisms left,but theremaybeacontamination revertedback
to the products. Therefore, it is required to increase the number of
microbes that can be detected bymicrobiology method. Under the
method,microorganism inUHTproductswill be incubatedat least 5
days.Thesecondstep is themicrobiological testing.Currently, there
The Notification of the Ministry of Public Health (No. 349), B.E.2556, Re: Methods, Equipment and Storages for
ManufacturingofThermallyProcessedLow-acidFoodsandAcidifiedFoodsPackaged inHermeticallySealedContainers
has regulated a control of UHT processing and finished products, while also stringently controlled of the production
quality under suitable self-monitoring system in order to help reduce and eliminate the hazards that contaminated to
consumers.
are two isolation methods including pour plate and streak plate. The
methods takemore than twodays forexamination;startwithpreparation,
inoculationanddilutionaswell as incubation,whichwill needmore than
7 days to report themicrobiological testing.
Testing InnovationwithRapidMethod
The rapidmicrobial detection system1 that usesATPBioluminescence
is an innovative technology and allows rapid assessment of UltraHigh
Temperature Processing (UHT) or Extended Shelf Life (ESL) milk and
sterilized dairy samples that have already been incubated within their
pack. With mentioned system, it will help reduce processes of
microbiological testing, which can reduce the duration of the test from
twodays to less than27minutes.Theoperatingsystemof theautomatic
instrumentmanages throughsoftware.The instrumentwillhaveaprocess
toeliminateATPof thesamplingproductbyusingATPaseenzyme.After
that thecontaminatedcellsofmicroorganismswill bebroken,whichwill
release ATP, and cause sudden light by a reaction between ATP and
LuciferinsubstrateswithLuciferaseenzymes.Finally, the last step is the
censorship of light power. All of those steps will be processed
automatically.
ข้อมูลเพิ่มเติม / Additional Information
1 3MMicrobial LuminescenceSystem II (MLS II) ระบบการทดสอบเชื้อจุลินทรี ย์โดยอาศัย
หลักการตรวจATP
1 3MMicrobial LuminescenceSystem II (MLS II) a rapidmicrobial detection system
that usesATPBioluminescence
ออกไปโดยใช้เอนไซม์ ATPaseจากนั้นจะท�ำให้เซลล์ของเชื้ อจุลินทรีย์ที่ปนเปื้อนแตกออกซึ่งจะปลดปล่อย
ATPออกมาและจะมีการท�ำให้เกิ ดแสงโดยการท�ำปฏิกิริยากันระหว่างATPกับซั บสเตรทLuciferin และ
เอนไซม์ Luciferaseและขั้นตอนสุดท้ายคื อการตรวจวัดพลังงานแสงที่เกิดขึ้นซึ่งทุกขั้นตอนที่กล่าวมานี้
เกิดขึ้นอย่างอัตโนมัติ
Innovation forRapidMethodof
UHTProduct Inspection
Pre-Incubation MicrobiologyTest
Pre-Incubation
1